TY - JOUR
T1 - β-Hydroxy-β-Methylbutyrate Free Acid Attenuates Oxidative Stress Induced by a Single Bout of Plyometric Exercise
AU - Arazi, Hamid
AU - Hosseini, Zahra
AU - Asadi, Abbas
AU - Ramirez-Campillo, Rodrigo
AU - Suzuki, Katsuhiko
N1 - Funding Information:
We gratefully acknowledge the volunteers involved in this study. We thank our laboratory team for their technical assistance with the immunoassay procedures. Funding. This study was partially supported by the Scientific Research (A) (15H01833) and the Strategic Research Foundation at Private Universities 2015–2019 (S1511017) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Publisher Copyright:
© Copyright © 2019 Arazi, Hosseini, Asadi, Ramirez-Campillo and Suzuki.
PY - 2019/6/25
Y1 - 2019/6/25
N2 - Purpose: The purpose of this study was to examine the effect of β-hydroxy-β methylbutyrate free acid (HMB-FA) ingestion on oxidative stress and leukocyte responses to plyometric exercise. Methods: In a randomized double-blind placebo-controlled design, physically active males were assigned to the HMB-FA (n = 8) or placebo (n = 8) groups that consumed either 1 g of HMB-FA or placebo 30 min prior to performing an acute plyometric exercise protocol (15 sets of 10 repetitions of maximal-effort vertical jumps). Blood was obtained pre-(T1), post-(T2), and 1-h post-(T3) exercise to determine changes in serum levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), protein carbonyl (PC), and white blood cells (WBC). Results: The exercise protocol significantly elevated 8-OHdG (HMB-FA, T2 9.5 and T3 12.6%; placebo, T2 18.2 and T3 36.5%), MDA (HMB-FA, T2 11.6 and T3 25.2%; placebo, T2 11.8 and T3 41%) and PC (HMB-FA, T2 6.9 and T3 25%; placebo, T2 23.4 and T3 55.3%) at post- and 1-h post-exercise, respectively. However, at 1-h post-exercise, greater increases in oxidative stress markers (8-OHdG 36.5 vs. 12.6%; MDA 41 vs. 25.1% and PC 55.3 vs. 25%) were observed in the placebo group compared to the HMB-FA group (p < 0.05). In addition, the WBC level was greater for the placebo group in comparison to the HMB-FA group at post-exercise. Conclusion: HMB-FA attenuated oxidative stress and leukocyte responses to plyometric exercise compared with placebo.
AB - Purpose: The purpose of this study was to examine the effect of β-hydroxy-β methylbutyrate free acid (HMB-FA) ingestion on oxidative stress and leukocyte responses to plyometric exercise. Methods: In a randomized double-blind placebo-controlled design, physically active males were assigned to the HMB-FA (n = 8) or placebo (n = 8) groups that consumed either 1 g of HMB-FA or placebo 30 min prior to performing an acute plyometric exercise protocol (15 sets of 10 repetitions of maximal-effort vertical jumps). Blood was obtained pre-(T1), post-(T2), and 1-h post-(T3) exercise to determine changes in serum levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), protein carbonyl (PC), and white blood cells (WBC). Results: The exercise protocol significantly elevated 8-OHdG (HMB-FA, T2 9.5 and T3 12.6%; placebo, T2 18.2 and T3 36.5%), MDA (HMB-FA, T2 11.6 and T3 25.2%; placebo, T2 11.8 and T3 41%) and PC (HMB-FA, T2 6.9 and T3 25%; placebo, T2 23.4 and T3 55.3%) at post- and 1-h post-exercise, respectively. However, at 1-h post-exercise, greater increases in oxidative stress markers (8-OHdG 36.5 vs. 12.6%; MDA 41 vs. 25.1% and PC 55.3 vs. 25%) were observed in the placebo group compared to the HMB-FA group (p < 0.05). In addition, the WBC level was greater for the placebo group in comparison to the HMB-FA group at post-exercise. Conclusion: HMB-FA attenuated oxidative stress and leukocyte responses to plyometric exercise compared with placebo.
KW - free radicals
KW - leucine
KW - oxidative stress
KW - plyometric training
KW - stretch-shortening cycle
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U2 - 10.3389/fphys.2019.00776
DO - 10.3389/fphys.2019.00776
M3 - Article
AN - SCOPUS:85068444997
SN - 1664-042X
VL - 10
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 776
ER -