TY - JOUR
T1 - 16s rRNA-targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles
AU - Matsunaga, Tadashi
AU - Takeyama, Haruko
AU - Nakayama, Hideki
N1 - Funding Information:
This work was partially supported by a Grant-in Aid for Scientific Research on Priority Areas (A) no. 10145102, from the Ministry of Education, Science, Sports and Culture.
PY - 2001
Y1 - 2001
N2 - 16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was performed by a sandwich hybridization using the capture probes - BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.
AB - 16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was performed by a sandwich hybridization using the capture probes - BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.
KW - 16S rRNA
KW - Bacterial magnetic particles(BMPs)
KW - Cyanobacteria
KW - Identification
KW - Magnetic-capture hybridization
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U2 - 10.1023/A:1017990518648
DO - 10.1023/A:1017990518648
M3 - Article
AN - SCOPUS:0034824539
SN - 0921-8971
VL - 13
SP - 389
EP - 394
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 4
ER -