3D reconstruction of internal structure of animal body using near-infrared light

Trung Nghia Tran, Kohei Yamamoto, Takeshi Namita, Yuji Kato, Koichi Shimizu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse's abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.

Original languageEnglish
Title of host publicationBiomedical Applications of Light Scattering VIII
ISBN (Print)9780819498656
Publication statusPublished - 2014
Externally publishedYes
EventBiomedical Applications of Light Scattering VIII - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 3

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherBiomedical Applications of Light Scattering VIII
Country/TerritoryUnited States
CitySan Francisco, CA


  • 3D optical imaging
  • 3D reconstruction
  • Bio-imaging
  • Diffuse optical tomography
  • Point spread function
  • Scattering suppression
  • Small animal imaging
  • Transillumination image

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


Dive into the research topics of '3D reconstruction of internal structure of animal body using near-infrared light'. Together they form a unique fingerprint.

Cite this