TY - JOUR
T1 - 7-methylguanosine at the anticodon wobble position of squid mitochondrial tRNA(Ser)GCU
T2 - Molecular basis for assignment of AGA/AGG codons as serine in invertebrate mitochondria
AU - Tomita, Kozo
AU - Ueda, Takuya
AU - Watanabe, Kimitsuna
N1 - Funding Information:
We thank Mr. T. Kaifu for showing us the unpublished partial sequence of squid mt DNA, Dr. S. Yokobori (at present, Tokyo University of Pharmacy and Life Science) for data analysis, Dr. Y. Watanabe (at present, Dalhousie University, Canada) for valuable discussion, and Mr. H. Nambu (at present, Banyu Pharmaceutical Co.) for continuous encouragement. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan and by the Human Frontier Science Program Organization.
PY - 1998/7/30
Y1 - 1998/7/30
N2 - In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).
AB - In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).
KW - (Squid, Loligo bleekeri)
KW - 7- Methylguanosine
KW - Decoding of AGA and AGG codons
KW - Mitochondrion
KW - TRNA(Ser)GCU
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U2 - 10.1016/S0167-4781(98)00099-2
DO - 10.1016/S0167-4781(98)00099-2
M3 - Article
C2 - 9714749
AN - SCOPUS:0032581682
SN - 0167-4781
VL - 1399
SP - 78
EP - 82
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1
ER -