A catalytic immuno-reactor for the amperometric determination of human serum albumin

Isao Karube; Tadashi Matsunaga; Takeru Satoh; Shuichi Suzuki

doi:10.1016/S0003-2670(00)85560-9

A catalytic immuno-reactor for the amperometric determination of human serum albumin

Isao Karube^*, Tadashi Matsunaga, Takeru Satoh, Shuichi Suzuki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml^-1.

Original language	English
Pages (from-to)	283-287
Number of pages	5
Journal	Analytica Chimica Acta
Volume	156
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2670(00)85560-9
Publication status	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Analytical Chemistry
Spectroscopy
Environmental Chemistry

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10.1016/S0003-2670(00)85560-9

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abstract = "A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.",

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AU - Karube, Isao

AU - Matsunaga, Tadashi

AU - Satoh, Takeru

AU - Suzuki, Shuichi

PY - 1984

Y1 - 1984

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AB - A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

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ER -

A catalytic immuno-reactor for the amperometric determination of human serum albumin

Abstract

ASJC Scopus subject areas

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