A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi; Kazuaki Sawaki; Atsushi Murata; Shinji Takeoka

doi:10.1039/c4cc09947h

A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi, Kazuaki Sawaki, Atsushi Murata, Shinji Takeoka^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

Original language	English
Pages (from-to)	7879-7882
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	37
DOIs	https://doi.org/10.1039/c4cc09947h
Publication status	Published - 2015 May 7

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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AU - Yamagishi, Kento

AU - Sawaki, Kazuaki

AU - Murata, Atsushi

AU - Takeoka, Shinji

N1 - Publisher Copyright: © The Royal Society of Chemistry 2015.

PY - 2015/5/7

Y1 - 2015/5/7

N2 - We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

AB - We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

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JO - Chemical Communications

JF - Chemical Communications

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ER -

A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Abstract

ASJC Scopus subject areas

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