A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions

Yukako Hihara*, Masayuki Muramatsu, Kinu Nakamura, Kintake Sonoike

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEBS Letters
Issue number1-3
Publication statusPublished - 2004 Sept 10
Externally publishedYes


  • DIG, digoxigenin
  • DNA microarray
  • IPTG, isopropyl-β-D(-) -thiogalactopyranoside
  • LysR family
  • PCR, polymerase chain reaction
  • Pirin
  • Salt stress
  • Synechocystis sp. PCC 6803
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions'. Together they form a unique fingerprint.

Cite this