A DIGE proteomic analysis for low-intensity exercise-trained rat skeletal muscle

Wataru Yamaguchi*, Eri Takai, Mitsuru Higuchi, Izumi Tabata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Background: Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. Purpose: Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). Methods: For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. Results: Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Conclusion: The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsyna, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalJapanese Journal of Physical Fitness and Sports Medicine
Issue number5
Publication statusPublished - 2011


  • Epitrochlearis
  • Low-intensity exercise training
  • Proteomics
  • Swimming

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation


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