A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions

Shota Yamamoto; Tatsuya Miyama; Takafumi Komoda; Michiko Sugawara; Makiko Nonomura; Jun Nakanishi

doi:10.2116/analsci.19P233

A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions

Shota Yamamoto, Tatsuya Miyama, Takafumi Komoda, Michiko Sugawara, Makiko Nonomura, Jun Nakanishi^*

^*Corresponding author for this work

Graduate School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Epithelial-mesenchymal transition (EMT), a qualitative change in cell migration behavior during cancer invasion and metastasis, is becoming a new target for anticancer drugs. Therefore, it is crucial to develop in vitro assays for the evaluation of the abilities of drug candidates to control EMT progression. We herein report on a method for the quantification of the EMT based on particle image velocimetry and correlation functions. The exponential fitting of the correlation curve gives an index (λ), which represents transforming growth factor (TGF)-β1-induced EMT progression and its suppression by inhibitors. Moreover, real-time monitoring of the λ value illustrates a time-dependent EMT progressing process, which occurs earlier than the bio-chemical changes in an EMT marker protein expression. The results demonstrate the usefulness of the present method for kinetic studies of EMT progression as well as EMT inhibitor screening.

Original language	English
Pages (from-to)	263-268
Number of pages	6
Journal	Analytical Sciences
Volume	36
Issue number	2
DOIs	https://doi.org/10.2116/analsci.19P233
Publication status	Published - 2020

Keywords

Cancer
Cell migration
Drug screening
Epithelial-mesenchymal transition
Mathematical analysis
Particle image velocimetry

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/analsci.19P233

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title = "A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions",

abstract = "Epithelial-mesenchymal transition (EMT), a qualitative change in cell migration behavior during cancer invasion and metastasis, is becoming a new target for anticancer drugs. Therefore, it is crucial to develop in vitro assays for the evaluation of the abilities of drug candidates to control EMT progression. We herein report on a method for the quantification of the EMT based on particle image velocimetry and correlation functions. The exponential fitting of the correlation curve gives an index (λ), which represents transforming growth factor (TGF)-β1-induced EMT progression and its suppression by inhibitors. Moreover, real-time monitoring of the λ value illustrates a time-dependent EMT progressing process, which occurs earlier than the bio-chemical changes in an EMT marker protein expression. The results demonstrate the usefulness of the present method for kinetic studies of EMT progression as well as EMT inhibitor screening.",

keywords = "Cancer, Cell migration, Drug screening, Epithelial-mesenchymal transition, Mathematical analysis, Particle image velocimetry",

author = "Shota Yamamoto and Tatsuya Miyama and Takafumi Komoda and Michiko Sugawara and Makiko Nonomura and Jun Nakanishi",

note = "Funding Information: This study was supported in part by the Japan Society for the Promotion of Science (JSPS), Kakenhi (No. 15H03831, 16K05523, 18H02010). We thank Kenji Sugiyama (NIMS) for the experimental support and Takumi Hanzawa (Chiba Univ.) for the valuable advice on the PIV analysis. Publisher Copyright: {\textcopyright} The Japan Society for Analytical Chemistry.",

year = "2020",

doi = "10.2116/analsci.19P233",

language = "English",

volume = "36",

pages = "263--268",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Japan Society for Analytical Chemistry",

number = "2",

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TY - JOUR

T1 - A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions

AU - Yamamoto, Shota

AU - Miyama, Tatsuya

AU - Komoda, Takafumi

AU - Sugawara, Michiko

AU - Nonomura, Makiko

AU - Nakanishi, Jun

N1 - Funding Information: This study was supported in part by the Japan Society for the Promotion of Science (JSPS), Kakenhi (No. 15H03831, 16K05523, 18H02010). We thank Kenji Sugiyama (NIMS) for the experimental support and Takumi Hanzawa (Chiba Univ.) for the valuable advice on the PIV analysis. Publisher Copyright: © The Japan Society for Analytical Chemistry.

PY - 2020

Y1 - 2020

N2 - Epithelial-mesenchymal transition (EMT), a qualitative change in cell migration behavior during cancer invasion and metastasis, is becoming a new target for anticancer drugs. Therefore, it is crucial to develop in vitro assays for the evaluation of the abilities of drug candidates to control EMT progression. We herein report on a method for the quantification of the EMT based on particle image velocimetry and correlation functions. The exponential fitting of the correlation curve gives an index (λ), which represents transforming growth factor (TGF)-β1-induced EMT progression and its suppression by inhibitors. Moreover, real-time monitoring of the λ value illustrates a time-dependent EMT progressing process, which occurs earlier than the bio-chemical changes in an EMT marker protein expression. The results demonstrate the usefulness of the present method for kinetic studies of EMT progression as well as EMT inhibitor screening.

AB - Epithelial-mesenchymal transition (EMT), a qualitative change in cell migration behavior during cancer invasion and metastasis, is becoming a new target for anticancer drugs. Therefore, it is crucial to develop in vitro assays for the evaluation of the abilities of drug candidates to control EMT progression. We herein report on a method for the quantification of the EMT based on particle image velocimetry and correlation functions. The exponential fitting of the correlation curve gives an index (λ), which represents transforming growth factor (TGF)-β1-induced EMT progression and its suppression by inhibitors. Moreover, real-time monitoring of the λ value illustrates a time-dependent EMT progressing process, which occurs earlier than the bio-chemical changes in an EMT marker protein expression. The results demonstrate the usefulness of the present method for kinetic studies of EMT progression as well as EMT inhibitor screening.

KW - Cancer

KW - Cell migration

KW - Drug screening

KW - Epithelial-mesenchymal transition

KW - Mathematical analysis

KW - Particle image velocimetry

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JO - Analytical Sciences

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A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions

Abstract

Keywords

ASJC Scopus subject areas

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