A method for evaluating nucleosome stability with a protein-binding fluorescent dye

Hiroyuki Taguchi; Naoki Horikoshi; Yasuhiro Arimura; Hitoshi Kurumizaka

doi:10.1016/j.ymeth.2014.08.019

A method for evaluating nucleosome stability with a protein-binding fluorescent dye

Hiroyuki Taguchi, Naoki Horikoshi, Yasuhiro Arimura, Hitoshi Kurumizaka^*

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.

Original language	English
Pages (from-to)	119-126
Number of pages	8
Journal	Methods
Volume	70
Issue number	2-3
DOIs	https://doi.org/10.1016/j.ymeth.2014.08.019
Publication status	Published - 2014

Keywords

Chromatin
Histone octamer
Histone variant
Nucleosome
SYPRO Orange
Tetrasome

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ymeth.2014.08.019

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title = "A method for evaluating nucleosome stability with a protein-binding fluorescent dye",

abstract = "Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.",

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AU - Taguchi, Hiroyuki

AU - Horikoshi, Naoki

AU - Arimura, Yasuhiro

AU - Kurumizaka, Hitoshi

PY - 2014

Y1 - 2014

N2 - Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.

AB - Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.

KW - Chromatin

KW - Histone octamer

KW - Histone variant

KW - Nucleosome

KW - SYPRO Orange

KW - Tetrasome

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A method for evaluating nucleosome stability with a protein-binding fluorescent dye

Abstract

Keywords

ASJC Scopus subject areas

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