TY - GEN
T1 - A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters
AU - Isokawa, Muneki
AU - Nakanishi, Kanki
AU - Kanamori, Takahiro
AU - Zhuang, Huiqi
AU - Yamazaki, Hayate
AU - Sugaya, Takuo
AU - Yoon, Dong Hyun
AU - Sekiguchi, Tetsushi
AU - Funatsu, Takashi
AU - Shoji, Shuichi
AU - Tsunoda, Makoto
N1 - Funding Information:
This research was partially supported by JSPS KAKENHI Grant Numbers JP17K08234, JP16K14705, JP16H01419, JP15J12103; JSPS Bilateral Open Partnership Joint Research Projects Program; MEXT KAKENHI Grant Number JP16H02349; and the Center of Innovation Program from the Japan Science and Technology Agency (JST).
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - This work describes an on-chip mixer for post-column derivatization after liquid chromatography (LC) separation. The mixer was developed as a microchannel filled with a diamond-shaped pillar array (pillar array mixer, PA-mixer), which simultaneously enhanced the derivatization reaction and prevented the diffusion of the separated analytes. By utilizing the LC chip with a PA-mixer, neurotransmitters that are otherwise undetectable without derivatization could be separated, derivatized with a fluorogenic reagent, and detected within 15 s. In this manner, an automated bioanalytical method for point-of-care diagnosis was made possible by using the LC chip.
AB - This work describes an on-chip mixer for post-column derivatization after liquid chromatography (LC) separation. The mixer was developed as a microchannel filled with a diamond-shaped pillar array (pillar array mixer, PA-mixer), which simultaneously enhanced the derivatization reaction and prevented the diffusion of the separated analytes. By utilizing the LC chip with a PA-mixer, neurotransmitters that are otherwise undetectable without derivatization could be separated, derivatized with a fluorogenic reagent, and detected within 15 s. In this manner, an automated bioanalytical method for point-of-care diagnosis was made possible by using the LC chip.
KW - Catecholamine
KW - Diagnosis
KW - Fluorogenic derivatization
KW - Separation
UR - http://www.scopus.com/inward/record.url?scp=85079638796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079638796&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079638796
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 1318
EP - 1319
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -