l-Amino acid α-ligase (EC 18.104.22.168) catalyzed formation of α-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.
- Bacillus licheniformis
- enzymatic method
- l-amino acid
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology