A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

Toshinobu Arai; Kuniki Kino

doi:10.1271/bbb.80439

A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

Toshinobu Arai, Kuniki Kino^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPP_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various heterodipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.

Original language	English
Pages (from-to)	3048-3050
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	11
DOIs	https://doi.org/10.1271/bbb.80439
Publication status	Published - 2008

Keywords

L-amino acid ligase
Peptide synthesis
Phaseolotoxin

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.80439

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title = "A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A",

abstract = "In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPP_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various heterodipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.",

keywords = "L-amino acid ligase, Peptide synthesis, Phaseolotoxin",

author = "Toshinobu Arai and Kuniki Kino",

note = "Funding Information: We thank Dr. Kazuhiko Tabata of Kyowa Hakko Kogyo Co., Ltd., for helpful discussion. This work was financially supported in part by the Global COE program of the MEXT Center for Practical Chemical Wisdom and in part by a Waseda University Grant for Special Research, Project 2008B-186.",

year = "2008",

doi = "10.1271/bbb.80439",

language = "English",

volume = "72",

pages = "3048--3050",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "11",

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T1 - A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

AU - Arai, Toshinobu

AU - Kino, Kuniki

N1 - Funding Information: We thank Dr. Kazuhiko Tabata of Kyowa Hakko Kogyo Co., Ltd., for helpful discussion. This work was financially supported in part by the Global COE program of the MEXT Center for Practical Chemical Wisdom and in part by a Waseda University Grant for Special Research, Project 2008B-186.

PY - 2008

Y1 - 2008

N2 - In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPP_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various heterodipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.

AB - In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPP_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various heterodipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.

KW - L-amino acid ligase

KW - Peptide synthesis

KW - Phaseolotoxin

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SN - 0916-8451

VL - 72

SP - 3048

EP - 3050

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 11

ER -

A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

Abstract

Keywords

ASJC Scopus subject areas

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