TY - JOUR
T1 - A novel small protein of Bacillus subtilis involved in spore germination and spore coat assembly
AU - Kodama, Takeko
AU - Matsubayashi, Takeshi
AU - Yanagihara, Tadayoshi
AU - Komoto, Hiroyuki
AU - Ara, Katsutoshi
AU - Ozaki, Katsuya
AU - Kuwana, Ritsuko
AU - Imamura, Daisuke
AU - Takamatsu, Hiromu
AU - Watabe, Kazuhito
AU - Sekiguchi, Junichi
N1 - Funding Information:
We would like to thank Dr. Masakuni Serizawa and Dr. Tatsuya Fukushima for technical advice, and Ms. Nozomi Hayashi for technical assistance. This research was carried out as a part of the Project for the Development of a Technological Infrastructure for Industrial Bioprocesses through R and D on New Industrial Science and Technology Frontiers of the Ministry of Economy, Trade, and Industry (METI), and was supported by the New Energy and Industrial Technology Development Organization (NEDO). It was also supported by Grants-in-Aid for Scientific Research on Priority Areas, Genome Biology (no. 12206005), by the Global COE Program, and by Scientific Research (B) (no. 16380059) and (A) (no. 22248008 to J. S.) of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2011
Y1 - 2011
N2 - Two small genes named sscA (previously yhzE) and orf-62, located in the prsA-yhaK intergenic region of the Bacillus subtilis genome, were transcribed by SigK and GerE in the mother cells during the later stages of sporulation. The SscA-FLAG fusion protein was produced from T5 of sporulation and incorporated into mature spores. sscA mutant spores exhibited poor germination, and Tricine-SDS-PAGE analysis showed that the coat protein profile of the mutant differed from that of the wild type. Bands corresponding to proteins at 59, 36, 5, and 3 kDa were reduced in the sscA null mutant. Western blot analysis of anti-CotB and anti-CotG antibodies showed reductions of the proteins at 59 kDa and 36 kDa in the sscA mutant spores. These proteins correspond to CotB and CotG. By immunoblot analysis of an anti-CotH antibody, we also observed that CotH was markedly reduced in the sscA mutant spores. It appears that SscA is a novel spore protein involved in the assembly of several components of the spore coat, including CotB, CotG, and CotH, and is associated with spore germination.
AB - Two small genes named sscA (previously yhzE) and orf-62, located in the prsA-yhaK intergenic region of the Bacillus subtilis genome, were transcribed by SigK and GerE in the mother cells during the later stages of sporulation. The SscA-FLAG fusion protein was produced from T5 of sporulation and incorporated into mature spores. sscA mutant spores exhibited poor germination, and Tricine-SDS-PAGE analysis showed that the coat protein profile of the mutant differed from that of the wild type. Bands corresponding to proteins at 59, 36, 5, and 3 kDa were reduced in the sscA null mutant. Western blot analysis of anti-CotB and anti-CotG antibodies showed reductions of the proteins at 59 kDa and 36 kDa in the sscA mutant spores. These proteins correspond to CotB and CotG. By immunoblot analysis of an anti-CotH antibody, we also observed that CotH was markedly reduced in the sscA mutant spores. It appears that SscA is a novel spore protein involved in the assembly of several components of the spore coat, including CotB, CotG, and CotH, and is associated with spore germination.
KW - Bacillus subtilis
KW - Germination
KW - Small protein
KW - Spore coat
KW - sscA
UR - http://www.scopus.com/inward/record.url?scp=79959558676&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959558676&partnerID=8YFLogxK
U2 - 10.1271/bbb.110029
DO - 10.1271/bbb.110029
M3 - Article
C2 - 21670523
AN - SCOPUS:79959558676
SN - 0916-8451
VL - 75
SP - 1119
EP - 1128
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -