A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells

Mitsumasa Homma; Yoshiaki Takei; Atsushi Murata; Takafumi Inoue; Shinji Takeoka

doi:10.1039/c4cc10349a

A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells

Mitsumasa Homma, Yoshiaki Takei, Atsushi Murata, Takafumi Inoue, Shinji Takeoka^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.

Original language	English
Pages (from-to)	6194-6197
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	28
DOIs	https://doi.org/10.1039/c4cc10349a
Publication status	Published - 2015 Apr 11

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.",

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AU - Homma, Mitsumasa

AU - Takei, Yoshiaki

AU - Murata, Atsushi

AU - Inoue, Takafumi

AU - Takeoka, Shinji

N1 - Publisher Copyright: © The Royal Society of Chemistry 2015.

PY - 2015/4/11

Y1 - 2015/4/11

N2 - Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.

AB - Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.

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A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells

Abstract

ASJC Scopus subject areas

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