A simple means to immobilize enzyme into conducting polymers via entrapment

Jun Chen; Bjorn Winther Jensen; Carol Lynam; Orawan Ngamna; Simon Moulton; Weimin Zhang; Gordon G. Wallace

doi:10.1149/1.2201306

A simple means to immobilize enzyme into conducting polymers via entrapment

Jun Chen^*, Bjorn Winther Jensen, Carol Lynam, Orawan Ngamna, Simon Moulton, Weimin Zhang, Gordon G. Wallace

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

Original language	English
Journal	Electrochemical and Solid-State Letters
Volume	9
Issue number	7
DOIs	https://doi.org/10.1149/1.2201306
Publication status	Published - 2006 Jul
Externally published	Yes

ASJC Scopus subject areas

Electrochemistry
Materials Science(all)

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title = "A simple means to immobilize enzyme into conducting polymers via entrapment",

abstract = "Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.",

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T1 - A simple means to immobilize enzyme into conducting polymers via entrapment

AU - Chen, Jun

AU - Winther Jensen, Bjorn

AU - Lynam, Carol

AU - Ngamna, Orawan

AU - Moulton, Simon

AU - Zhang, Weimin

AU - Wallace, Gordon G.

PY - 2006/7

Y1 - 2006/7

N2 - Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

AB - Poly(3,4-ethylenedioxy thiophene) (PEDT), obtained by chemical vapor phase polymerization can shrink to 5% of its original thickness during a washing step. This phenomenon was exploited to immobilize the active enzyme, horseradish peroxidase (HRP), into PEDT films by rinsing with an ethanol solution containing HRP. The use of the PEDT-HRP as an amperometric biosensor was demonstrated by sensing 5 mM H2 O2 in phosphate buffered saline solution with a sensitivity of 190 μA cm-2. The stability of the immobilization of HRP into the ''stuffed'' PEDT matrix was also characterized by repeated amperometric detection and scanning electron microscopy mapping images obtained before and after exposure to H2 O2.

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A simple means to immobilize enzyme into conducting polymers via entrapment

Abstract

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