Acquirement of hairpin ribozyme activity by the long substrate-binding site.

S. Hisamatsu*, S. Sonoki, Yo Kikuchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Three hairpin ribozymes were designed to cleave Escherichia coli beta-glucuronidase (GUS) mRNA at a target site. Two of the designed ribozymes (HG10L and HG10L2) had long substrate binding sites and the other (HG10) had short substrate binding site. All three ribozymes cleaved the substrate, and HG10L and HG10L2 cleaved it efficiently. However, HG10 had very low activity. Effect of length of substrate binding site of the ribozyme will be discussed.

Original languageEnglish
Pages (from-to)173-174
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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