Active species for Ce(IV)-induced hydrolysis of phosphodiester linkage in cAMP and DNA

Jun Sumaoka, Kenichiro Furuki, Yuki Kojima, Masahiko Shibata, Kimihiko Hirao, Naoya Takeda, Makoto Komiyama*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The hydrolysis of cyclic adenosine 3′,5′-monophosphate and 2′-deoxythymidylyl(3′-5′)2′-deoxythymidine by Ce(NH 4)2(NO3)6 was kinetically studied. The rate of hydrolysis was fairly proportional to the concentration of [Ce 2IV (OH)4]4+, showing that this is the catalytically active species. According to quantum-chemical calculation, the two Ce(IV) ions in this [Ce2IV (OH)4] 4+ cluster are bridged by two OH residues. Upon the complex formation with H2PO4- (a model compound for the phosphodiesters), these two Ce(IV) ions bind the two oxygen atoms of the substrate and enhance the electrophilicity of the phosphorus atom. The catalytic mechanism of Ce(IV)-induced hydrolysis of phosphodiesters has been proposed on the basis these results.

Original languageEnglish
Pages (from-to)523-538
Number of pages16
JournalNucleosides, Nucleotides and Nucleic Acids
Issue number4-6
Publication statusPublished - 2006 Jun 1
Externally publishedYes


  • Artificial nuclease
  • Cerium
  • DNA cleavage
  • Enzyme models
  • Phosphodiester hydrolysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics


Dive into the research topics of 'Active species for Ce(IV)-induced hydrolysis of phosphodiester linkage in cAMP and DNA'. Together they form a unique fingerprint.

Cite this