Activin A increases the number of follicle-stimulating hormone cells in anterior pituitary cultures

Tetsuro Katayama, Kunio Shiota, Michio Takahashi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


The mode of action of activin A on the anterior pituitary gland (AP) was investigated using primary cultured cells. The AP cells were cultured with activin A (1 ng/ml) at various cell densities for 24-96 h, and further incubated for 3 h with activin A-free medium. When the cells were pretreated with activin A for 48 h, follicle-stimulating hormone (FSH) secretion during the following 3-h incubation was increased only at a density of 1 × 105 cells/200 mm2, but not at 2 × 105 or 4 × 105 cells/200 mm2. A longer pretreatment (96 h) was required in order to induce this response at a density of 2 × 105 cells/200 mm2. Luteinizing hormone (LH) secretion was not affected by activin A. Thus, the FSH secretory activity of the primary culture of AP was stimulated by activin A in a cell density-dependent manner. Furthermore, it was found that treatment with activin A (10 ng/ml) for 72 h increased the number of immunoreactive FSH cells by 25-41%, and that these newly induced FSH cells were low responders to gonadotropin-releasing hormone stimulation. The proportions of immunoreactive LH, thyroid-stimulating hormone, prolactin or growth hormone cells were not affected. From these results, we conclude that activin A increases FSH secretion by changing the cell population of pituitary gonadotropes.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalMolecular and Cellular Endocrinology
Issue number2-3
Publication statusPublished - 1990 Mar 5
Externally publishedYes


  • Activin A
  • Anterior pituitary
  • Cell density
  • Cell number

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism


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