TY - JOUR
T1 - ADF/cofilin is not essential but is critically important for actin activities during phagocytosis in Tetrahymena thermophila
AU - Shiozaki, Nanami
AU - Nakano, Kentaro
AU - Kushida, Yasuharu
AU - Noguchi, Taro Q.P.
AU - Uyeda, Taro Q.P.
AU - Wloga, Dorota
AU - Dave, Drashti
AU - Vasudevan, Krishna Kumar
AU - Gaertig, Jacek
AU - Numataa, Osamu
PY - 2013/8
Y1 - 2013/8
N2 - ADF/cofilin is a highly conserved actin-modulating protein. Reorganization of the actin cytoskeleton in vivo through severing and depolymerizing of F-actin by this protein is essential for various cellular events, such as endocytosis, phagocytosis, cytokinesis, and cell migration. We show that in the ciliate Tetrahymena thermophila, the ADF/cofilin homologue Adf73p associates with actin on nascent food vacuoles. Overexpression of Adf73p disrupted the proper localization of actin and inhibited the formation of food vacuoles. In vitro, recombinant Adf73p promoted the depolymerization of filaments made of T. thermophila actin (Act1p). Knockout cells lacking the ADF73 gene are viable but grow extremely slowly and have a severely decreased rate of food vacuole formation. Knockout cells have abnormal aggregates of actin in the cytoplasm. Surprisingly, unlike the case in animals and yeasts, in Tetrahymena, ADF/cofilin is not required for cytokinesis. Thus, the Tetrahymena model shows promise for future studies of the role of ADF/cofilin in vivo.
AB - ADF/cofilin is a highly conserved actin-modulating protein. Reorganization of the actin cytoskeleton in vivo through severing and depolymerizing of F-actin by this protein is essential for various cellular events, such as endocytosis, phagocytosis, cytokinesis, and cell migration. We show that in the ciliate Tetrahymena thermophila, the ADF/cofilin homologue Adf73p associates with actin on nascent food vacuoles. Overexpression of Adf73p disrupted the proper localization of actin and inhibited the formation of food vacuoles. In vitro, recombinant Adf73p promoted the depolymerization of filaments made of T. thermophila actin (Act1p). Knockout cells lacking the ADF73 gene are viable but grow extremely slowly and have a severely decreased rate of food vacuole formation. Knockout cells have abnormal aggregates of actin in the cytoplasm. Surprisingly, unlike the case in animals and yeasts, in Tetrahymena, ADF/cofilin is not required for cytokinesis. Thus, the Tetrahymena model shows promise for future studies of the role of ADF/cofilin in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84880811829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880811829&partnerID=8YFLogxK
U2 - 10.1128/EC.00074-13
DO - 10.1128/EC.00074-13
M3 - Article
C2 - 23729382
AN - SCOPUS:84880811829
SN - 1535-9778
VL - 12
SP - 1080
EP - 1086
JO - Eukaryotic Cell
JF - Eukaryotic Cell
IS - 8
ER -