Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

Daisuke Umeno; Takeshi Kano; Mizuo Maeda

doi:10.1016/S0003-2670(98)00094-4

Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

Daisuke Umeno, Takeshi Kano, Mizuo Maeda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Polyacrylamide hydrogels comprising double-stranded DNA were synthesized and applied as the absorbent for DNA-binding mutagenic molecules. Vinyl groups were immobilized on salmon sperm DNA via photoreaction between DNA and a vinyl derivative of psoralen. The resulting DNA macromonomer was fed into the polymerization system of acrylamide and N,N'-methylenebisacrylamide to give a hybrid hydrogel between DNA and polyacrylamide. Adsorption functions of the DNA hydrogel were studied for a variety of DNA-binding drugs and dyes. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	101-108
Number of pages	8
Journal	Analytica Chimica Acta
Volume	365
Issue number	1-3
DOIs	https://doi.org/10.1016/S0003-2670(98)00094-4
Publication status	Published - 1998 Jun 5
Externally published	Yes

Keywords

Affinity separation
Double-stranded DNA
Hybrid material
Mutagenic molecules
Polyacrylamide gel
Psoralen

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Environmental Chemistry

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10.1016/S0003-2670(98)00094-4

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title = "Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA",

abstract = "Polyacrylamide hydrogels comprising double-stranded DNA were synthesized and applied as the absorbent for DNA-binding mutagenic molecules. Vinyl groups were immobilized on salmon sperm DNA via photoreaction between DNA and a vinyl derivative of psoralen. The resulting DNA macromonomer was fed into the polymerization system of acrylamide and N,N'-methylenebisacrylamide to give a hybrid hydrogel between DNA and polyacrylamide. Adsorption functions of the DNA hydrogel were studied for a variety of DNA-binding drugs and dyes. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Affinity separation, Double-stranded DNA, Hybrid material, Mutagenic molecules, Polyacrylamide gel, Psoralen",

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note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Financial support by the Kumagai Foundation for Science and Technology is also acknowledged.",

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T1 - Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

AU - Umeno, Daisuke

AU - Kano, Takeshi

AU - Maeda, Mizuo

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Financial support by the Kumagai Foundation for Science and Technology is also acknowledged.

PY - 1998/6/5

Y1 - 1998/6/5

N2 - Polyacrylamide hydrogels comprising double-stranded DNA were synthesized and applied as the absorbent for DNA-binding mutagenic molecules. Vinyl groups were immobilized on salmon sperm DNA via photoreaction between DNA and a vinyl derivative of psoralen. The resulting DNA macromonomer was fed into the polymerization system of acrylamide and N,N'-methylenebisacrylamide to give a hybrid hydrogel between DNA and polyacrylamide. Adsorption functions of the DNA hydrogel were studied for a variety of DNA-binding drugs and dyes. Copyright (C) 1998 Elsevier Science B.V.

AB - Polyacrylamide hydrogels comprising double-stranded DNA were synthesized and applied as the absorbent for DNA-binding mutagenic molecules. Vinyl groups were immobilized on salmon sperm DNA via photoreaction between DNA and a vinyl derivative of psoralen. The resulting DNA macromonomer was fed into the polymerization system of acrylamide and N,N'-methylenebisacrylamide to give a hybrid hydrogel between DNA and polyacrylamide. Adsorption functions of the DNA hydrogel were studied for a variety of DNA-binding drugs and dyes. Copyright (C) 1998 Elsevier Science B.V.

KW - Affinity separation

KW - Double-stranded DNA

KW - Hybrid material

KW - Mutagenic molecules

KW - Polyacrylamide gel

KW - Psoralen

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Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

Abstract

Keywords

ASJC Scopus subject areas

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