An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa; Takeshi Kano; Chiharu Taga; Masahira Hattori; Yoshiyuki Sakaki; Harukazu Suzuki

doi:10.1016/0014-5793(93)81094-G

An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa, Takeshi Kano, Chiharu Taga, Masahira Hattori, Yoshiyuki Sakaki, Harukazu Suzuki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

Original language	English
Pages (from-to)	303-308
Number of pages	6
Journal	FEBS Letters
Volume	325
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)81094-G
Publication status	Published - 1993 Jul 5
Externally published	Yes

Keywords

Alzheimer's disease
Chromosome 21
Gene cloning
Neurofibromatosis 1
Plasmid vector
RNA splicing

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(93)81094-G

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abstract = "We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.",

keywords = "Alzheimer's disease, Chromosome 21, Gene cloning, Neurofibromatosis 1, Plasmid vector, RNA splicing",

author = "Naoki Ozawa and Takeshi Kano and Chiharu Taga and Masahira Hattori and Yoshiyuki Sakaki and Harukazu Suzuki",

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T1 - An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

AU - Ozawa, Naoki

AU - Kano, Takeshi

AU - Taga, Chiharu

AU - Hattori, Masahira

AU - Sakaki, Yoshiyuki

AU - Suzuki, Harukazu

PY - 1993/7/5

Y1 - 1993/7/5

N2 - We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

AB - We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

KW - Alzheimer's disease

KW - Chromosome 21

KW - Gene cloning

KW - Neurofibromatosis 1

KW - Plasmid vector

KW - RNA splicing

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JF - FEBS Letters

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An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Abstract

Keywords

ASJC Scopus subject areas

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