Analysis of calcium signals in steering neuronal growth cones in vitro

Hiroki Akiyama; Hiroyuki Kamiguchi

doi:10.1007/978-1-4939-0777-9_2

Analysis of calcium signals in steering neuronal growth cones in vitro

Hiroki Akiyama, Hiroyuki Kamiguchi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

Original language	English
Pages (from-to)	17-27
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1162
DOIs	https://doi.org/10.1007/978-1-4939-0777-9_2
Publication status	Published - 2014
Externally published	Yes

Keywords

Axon guidance
Ca2+ imaging
Chemotaxis
Growth cone
Turning assay

ASJC Scopus subject areas

Molecular Biology
Genetics
Medicine(all)

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10.1007/978-1-4939-0777-9_2

Cite this

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title = "Analysis of calcium signals in steering neuronal growth cones in vitro",

abstract = "Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.",

keywords = "Axon guidance, Ca2+ imaging, Chemotaxis, Growth cone, Turning assay",

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T1 - Analysis of calcium signals in steering neuronal growth cones in vitro

AU - Akiyama, Hiroki

AU - Kamiguchi, Hiroyuki

PY - 2014

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AB - Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

KW - Axon guidance

KW - Ca2+ imaging

KW - Chemotaxis

KW - Growth cone

KW - Turning assay

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JO - Methods in Molecular Biology

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ER -

Analysis of calcium signals in steering neuronal growth cones in vitro

Abstract

Keywords

ASJC Scopus subject areas

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