Analysis of DNA methylation by bisulfite reaction in neural cells as an example of orexin neurons

Koji Hayakawa; Mitsuko Hirosawa; Kunio Shiota

doi:10.1007/978-1-4939-2754-8_3

Analysis of DNA methylation by bisulfite reaction in neural cells as an example of orexin neurons

Koji Hayakawa, Mitsuko Hirosawa, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfi te-DNA reactions and combined bisulfi te restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.

Original language	English
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	55-63
Number of pages	9
Volume	105
DOIs	https://doi.org/10.1007/978-1-4939-2754-8_3
Publication status	Published - 2016
Externally published	Yes

Publication series

Name	Neuromethods
Volume	105
ISSN (Print)	08932336
ISSN (Electronic)	19406045

Keywords

Bisulfite reaction
Bisulfite sequence
COBRA
DNA methylation
Hypothalamus
Orexin

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Neuroscience(all)
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2754-8_3

Cite this

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abstract = "Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfi te-DNA reactions and combined bisulfi te restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.",

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language = "English",

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AU - Hayakawa, Koji

AU - Hirosawa, Mitsuko

AU - Shiota, Kunio

PY - 2016

Y1 - 2016

N2 - Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfi te-DNA reactions and combined bisulfi te restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.

AB - Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfi te-DNA reactions and combined bisulfi te restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.

KW - Bisulfite reaction

KW - Bisulfite sequence

KW - COBRA

KW - DNA methylation

KW - Hypothalamus

KW - Orexin

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DO - 10.1007/978-1-4939-2754-8_3

M3 - Chapter

AN - SCOPUS:84944463234

VL - 105

T3 - Neuromethods

SP - 55

EP - 63

BT - Neuromethods

PB - Humana Press Inc.

ER -

Analysis of DNA methylation by bisulfite reaction in neural cells as an example of orexin neurons

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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