Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

Toshimichi Shibue; Hisashi Kambe; Hiroyuki Nishide; Yoko Ohashi

doi:10.2116/analsci.11.793

Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

Toshimichi Shibue, Hisashi Kambe, Hiroyuki Nishide, Yoko Ohashi

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)^1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

Original language	English
Pages (from-to)	793-796
Number of pages	4
Journal	Analytical Sciences
Volume	11
Issue number	5
DOIs	https://doi.org/10.2116/analsci.11.793
Publication status	Published - 1995

Keywords

B(l—E)/E-constant linked-scan
B/E-constant linked-scan
collision-induced dissociation mass spectrometry/mass spectrometry
mass spectrometry
Porphyrin

ASJC Scopus subject areas

Analytical Chemistry

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title = "Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry",

abstract = "Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.",

keywords = "B(l—E)/E-constant linked-scan, B/E-constant linked-scan, collision-induced dissociation mass spectrometry/mass spectrometry, mass spectrometry, Porphyrin",

author = "Toshimichi Shibue and Hisashi Kambe and Hiroyuki Nishide and Yoko Ohashi",

year = "1995",

doi = "10.2116/analsci.11.793",

language = "English",

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AU - Shibue, Toshimichi

AU - Kambe, Hisashi

AU - Nishide, Hiroyuki

AU - Ohashi, Yoko

PY - 1995

Y1 - 1995

N2 - Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

AB - Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

KW - B(l—E)/E-constant linked-scan

KW - B/E-constant linked-scan

KW - collision-induced dissociation mass spectrometry/mass spectrometry

KW - mass spectrometry

KW - Porphyrin

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Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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