Analysis of histone modifications in neural cells as an example of orexin neurons

Koji Hayakawa, Chikako Yoneda, Ruiko Tani, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceedingChapter


Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Number of pages9
Publication statusPublished - 2016
Externally publishedYes

Publication series

ISSN (Print)08932336
ISSN (Electronic)19406045


  • Chromatin immunoprecipitation
  • Histone acetylation
  • Histone modification
  • Hypothalamus
  • Orexin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health


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