Analysis of histone modifications in neural cells as an example of orexin neurons

Koji Hayakawa; Chikako Yoneda; Ruiko Tani; Kunio Shiota

doi:10.1007/978-1-4939-2754-8_9

Analysis of histone modifications in neural cells as an example of orexin neurons

Koji Hayakawa, Chikako Yoneda, Ruiko Tani, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

Original language	English
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	139-147
Number of pages	9
Volume	105
DOIs	https://doi.org/10.1007/978-1-4939-2754-8_9
Publication status	Published - 2016
Externally published	Yes

Publication series

Name	Neuromethods
Volume	105
ISSN (Print)	08932336
ISSN (Electronic)	19406045

Keywords

Chromatin immunoprecipitation
Histone acetylation
Histone modification
Hypothalamus
Orexin

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Neuroscience(all)
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2754-8_9

Cite this

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AU - Hayakawa, Koji

AU - Yoneda, Chikako

AU - Tani, Ruiko

AU - Shiota, Kunio

PY - 2016

Y1 - 2016

N2 - Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

AB - Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

KW - Chromatin immunoprecipitation

KW - Histone acetylation

KW - Histone modification

KW - Hypothalamus

KW - Orexin

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DO - 10.1007/978-1-4939-2754-8_9

M3 - Chapter

AN - SCOPUS:84944463207

VL - 105

T3 - Neuromethods

SP - 139

EP - 147

BT - Neuromethods

PB - Humana Press Inc.

ER -

Analysis of histone modifications in neural cells as an example of orexin neurons

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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