Analysis of individual signaling complexes by mMAPS, a flow-proteometric system

Chao Kai Chou, Pei Hsiang Tsou, Jennifer L. Hsu, Heng Huan Lee, Ying Nai Wang, Jun Kameoka, Mien Chie Hung

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Signal transduction is essential for maintaining normal cell physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for multiparameter analysis of proteins in a single complex (mMAPS) for analyzing and quantifying individual target signaling complexes. mMAPS is a flow-proteometric system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissues or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe and requiring very low sample input.

Original languageEnglish
Article number20.11
JournalCurrent Protocols in Molecular Biology
Publication statusPublished - 2016 Apr 1
Externally publishedYes


  • Flow proteometry
  • Microfluidic
  • Protein-nucleic acid interaction
  • Protein-protein interaction
  • Single complex
  • Single molecule

ASJC Scopus subject areas

  • Molecular Biology


Dive into the research topics of 'Analysis of individual signaling complexes by mMAPS, a flow-proteometric system'. Together they form a unique fingerprint.

Cite this