Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306

Toru Kimura; Masato Iwatsuki; Yukihiro Asami; Aki Ishiyama; Rei Hokari; Kazuhiko Otoguro; Atsuko Matsumoto; Noriko Sato; Kazuro Shiomi; Yoko Takahashi; Satoshi Omura; Takuji Nakashima

doi:10.1038/ja.2016.28

Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306

Toru Kimura, Masato Iwatsuki, Yukihiro Asami, Aki Ishiyama, Rei Hokari, Kazuhiko Otoguro, Atsuko Matsumoto, Noriko Sato, Kazuro Shiomi, Yoko Takahashi^*, Satoshi Omura, Takuji Nakashima

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A new antibiotic, designated sagamilactam, was isolated from the cultured broth of Actinomadura sp. K13-0306 by Diaion HP-20, silica gel and octadecylsilane column chromatography followed by purification by HPLC. The chemical structure of the new compound was elucidated by spectroscopic analyses, including NMR and MS. The structure of sagamilactam proved to be a new compound consisting of a polyunsaturated and polyoxygenated 34-membered macrocyclic lactam containing diene, triene and tetraene conjugated olefins and a decalin moiety. Sagamilactam showed antitrypanosomal activity with an IC 50 value of 0.25±0.11 μM.

Original language	English
Pages (from-to)	818-824
Number of pages	7
Journal	Journal of Antibiotics
Volume	69
Issue number	11
DOIs	https://doi.org/10.1038/ja.2016.28
Publication status	Published - 2016 Nov 1
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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title = "Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306",

abstract = "A new antibiotic, designated sagamilactam, was isolated from the cultured broth of Actinomadura sp. K13-0306 by Diaion HP-20, silica gel and octadecylsilane column chromatography followed by purification by HPLC. The chemical structure of the new compound was elucidated by spectroscopic analyses, including NMR and MS. The structure of sagamilactam proved to be a new compound consisting of a polyunsaturated and polyoxygenated 34-membered macrocyclic lactam containing diene, triene and tetraene conjugated olefins and a decalin moiety. Sagamilactam showed antitrypanosomal activity with an IC 50 value of 0.25±0.11 μM.",

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T1 - Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306

AU - Kimura, Toru

AU - Iwatsuki, Masato

AU - Asami, Yukihiro

AU - Ishiyama, Aki

AU - Hokari, Rei

AU - Otoguro, Kazuhiko

AU - Matsumoto, Atsuko

AU - Sato, Noriko

AU - Shiomi, Kazuro

AU - Takahashi, Yoko

AU - Omura, Satoshi

AU - Nakashima, Takuji

PY - 2016/11/1

Y1 - 2016/11/1

N2 - A new antibiotic, designated sagamilactam, was isolated from the cultured broth of Actinomadura sp. K13-0306 by Diaion HP-20, silica gel and octadecylsilane column chromatography followed by purification by HPLC. The chemical structure of the new compound was elucidated by spectroscopic analyses, including NMR and MS. The structure of sagamilactam proved to be a new compound consisting of a polyunsaturated and polyoxygenated 34-membered macrocyclic lactam containing diene, triene and tetraene conjugated olefins and a decalin moiety. Sagamilactam showed antitrypanosomal activity with an IC 50 value of 0.25±0.11 μM.

AB - A new antibiotic, designated sagamilactam, was isolated from the cultured broth of Actinomadura sp. K13-0306 by Diaion HP-20, silica gel and octadecylsilane column chromatography followed by purification by HPLC. The chemical structure of the new compound was elucidated by spectroscopic analyses, including NMR and MS. The structure of sagamilactam proved to be a new compound consisting of a polyunsaturated and polyoxygenated 34-membered macrocyclic lactam containing diene, triene and tetraene conjugated olefins and a decalin moiety. Sagamilactam showed antitrypanosomal activity with an IC 50 value of 0.25±0.11 μM.

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Anti-trypanosomal compound, sagamilactam, a new polyene macrocyclic lactam from Actinomadura sp. K13-0306

Abstract

ASJC Scopus subject areas

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