Application of consistent massage-like perturbations on mouse calves and monitoring the resulting intramuscular pressure changes

Naoyoshi Sakitani, Takahiro Maekawa, Kumiko Saitou, Katsuhiko Suzuki, Shuhei Murase, Masakuni Tokunaga, Daisuke Yoshino, Keisuke Sawada, Atsushi Takashima, Motoshi Nagao, Toru Ogata, Yasuhiro Sawada*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Massage is generally recognized to be beneficial for relieving pain and inflammation. Although previous studies have reported anti-inflammatory effects of massage on skeletal muscles, the molecular mechanisms behind are poorly understood. We have recently developed a simple device to apply local cyclical compression (LCC), which can generate intramuscular pressure waves with varying amplitudes. Using this device, we have demonstrated that LCC modulates inflammatory responses of macrophages in situ and alleviates immobilization-induced muscle atrophy. Here, we describe protocols for the optimization and application of LCC as a massage-like intervention against immobilization-induced inflammation and atrophy of skeletal muscles of mouse hindlimbs. The protocol that we have developed can be useful for investigating the mechanism underlying beneficial effects of physical exercise and massage. Our experimental system provides a prototype of the analytical approach to elucidate the mechanical regulation of muscle homeostasis, although further development needs to be made for more comprehensive studies.

Original languageEnglish
Article numbere59475
JournalJournal of Visualized Experiments
Issue number151
Publication statusPublished - 2019


  • Disuse muscle atrophy
  • Immobilization
  • Immunology and Infection
  • Inflammation
  • Issue 151
  • Local cyclical compression
  • MCP-1
  • Macrophage
  • Massage

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


Dive into the research topics of 'Application of consistent massage-like perturbations on mouse calves and monitoring the resulting intramuscular pressure changes'. Together they form a unique fingerprint.

Cite this