Application of recombination transfer to the cognate Bacillus subtilis genome.

Satoshi Tomita*, Kenji Tsuge, Yo Kikuchi, Mitsuhiro Itaya

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Number of pages2
Publication statusPublished - 2003
Externally publishedYes


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