Ascorbic acid 2-phosphate enhances albumin mRNA expression and secretion of porcine hepatocytes

Dawei Yang; Toshie Koyama; Ai Okamura; Yoshiaki Shiba; Takayuki Akimoto; Makoto Kodama

doi:10.1016/j.msec.2003.12.015

Ascorbic acid 2-phosphate enhances albumin mRNA expression and secretion of porcine hepatocytes

Dawei Yang, Toshie Koyama, Ai Okamura, Yoshiaki Shiba, Takayuki Akimoto, Makoto Kodama^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. Asc2P enhanced the porcine albumin mRNA expression. The albumin secretion released into the culture medium from the porcine hepatocytes was also increased by Asc2P. However, Asc2P did not give any effects to rat hepatocytes on albumin mRNA expression and albumin secretion. This result shows that Asc2P is one of the important factors to enhance porcine hepatocyte activity.

Original language	English
Pages (from-to)	323-327
Number of pages	5
Journal	Materials Science and Engineering C
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.msec.2003.12.015
Publication status	Published - 2004 Apr 1
Externally published	Yes

Keywords

Albumin
Albumin secretion
Ascorbic acid 2-phosphate
Enzyme-linked immunosorbent assay
Messenger RNA expression
Porcine hepatocytes
Rat hepatocytes
Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

Medicine(all)

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10.1016/j.msec.2003.12.015

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title = "Ascorbic acid 2-phosphate enhances albumin mRNA expression and secretion of porcine hepatocytes",

abstract = "The effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. Asc2P enhanced the porcine albumin mRNA expression. The albumin secretion released into the culture medium from the porcine hepatocytes was also increased by Asc2P. However, Asc2P did not give any effects to rat hepatocytes on albumin mRNA expression and albumin secretion. This result shows that Asc2P is one of the important factors to enhance porcine hepatocyte activity.",

keywords = "Albumin, Albumin secretion, Ascorbic acid 2-phosphate, Enzyme-linked immunosorbent assay, Messenger RNA expression, Porcine hepatocytes, Rat hepatocytes, Reverse transcriptase-polymerase chain reaction",

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doi = "10.1016/j.msec.2003.12.015",

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T1 - Ascorbic acid 2-phosphate enhances albumin mRNA expression and secretion of porcine hepatocytes

AU - Yang, Dawei

AU - Koyama, Toshie

AU - Okamura, Ai

AU - Shiba, Yoshiaki

AU - Akimoto, Takayuki

AU - Kodama, Makoto

PY - 2004/4/1

Y1 - 2004/4/1

N2 - The effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. Asc2P enhanced the porcine albumin mRNA expression. The albumin secretion released into the culture medium from the porcine hepatocytes was also increased by Asc2P. However, Asc2P did not give any effects to rat hepatocytes on albumin mRNA expression and albumin secretion. This result shows that Asc2P is one of the important factors to enhance porcine hepatocyte activity.

AB - The effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. Asc2P enhanced the porcine albumin mRNA expression. The albumin secretion released into the culture medium from the porcine hepatocytes was also increased by Asc2P. However, Asc2P did not give any effects to rat hepatocytes on albumin mRNA expression and albumin secretion. This result shows that Asc2P is one of the important factors to enhance porcine hepatocyte activity.

KW - Albumin

KW - Albumin secretion

KW - Ascorbic acid 2-phosphate

KW - Enzyme-linked immunosorbent assay

KW - Messenger RNA expression

KW - Porcine hepatocytes

KW - Rat hepatocytes

KW - Reverse transcriptase-polymerase chain reaction

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DO - 10.1016/j.msec.2003.12.015

M3 - Article

AN - SCOPUS:1842679468

SN - 0928-4931

VL - 24

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EP - 327

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

IS - 3

ER -

Ascorbic acid 2-phosphate enhances albumin mRNA expression and secretion of porcine hepatocytes

Abstract

Keywords

ASJC Scopus subject areas

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