Assessment of antioxidant and anti-inflammatory actions of curcumin by application of novel neutrophil activity measurement system

Katsuhiko Suzuki*, Miki Tomari, Masaki Takahashi, Kaoru Sugama, Yoshihiko Otsuka, Atsushi Imaizumi, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: Neutrophils not only play a critical role in host defense by migrating to the site of infection and producing reactive oxygen species (ROS), but also mediate pathological process in tissue destruction and inflammatory diseases. Therefore, it is important to assess and modulate neutrophil activities. In the present study, we examined the effects of curcumin on migratory activity of neutrophils into the hydrogel and their production of ROS by newly-developed neutrophil activity measurement system. Method: Curcumin was diluted to medium in wide-range concentrations. Each solution was added on hydrogel, and the mixture of blood and luminol was layered on it in each tube. The cell count in the hydrogel was quantified as migratory activity of neutrophils, whereas the ROS production was measured by luminol-dependent chemiluminescence. Result: The migratory activity of neutrophils was significantly inhibited at 160 μg/mL only in the subgroup with higher migratory activity, whereas no effect was observed in the subgroup with lower migratory activity. ROS production of neutrophils was significantly inhibited at 20 μg/mL of curcumin in a concentration-dependent manner. Conclusion: This new method for measuring neutrophil activities can be applied not only for assessing antioxidant activity for reducing oxidative stress, but also anti-inflammatory activity in terms of reducing neutrophil migration. Curcumin was the most effective antioxidant among the tested plant extracts in our previous studies.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalJapanese Journal of Clinical Chemistry
Issue number4
Publication statusPublished - 2012 Oct 1


  • Anti-inflammatory action
  • Antioxidant action
  • Curcumin
  • Neutrophils
  • Reactive oxygen species

ASJC Scopus subject areas

  • Clinical Biochemistry


Dive into the research topics of 'Assessment of antioxidant and anti-inflammatory actions of curcumin by application of novel neutrophil activity measurement system'. Together they form a unique fingerprint.

Cite this