Astaxanthin production in a model cyanobacterium synechocystis sp. PCC 6803

Naoya Shimada; Yukiko Okuda; Kaisei Maeda; Daisuke Umeno; Shinichi Takaichi; Masahiko Ikeuchi

doi:10.2323/jgam.2020.01.003

Astaxanthin production in a model cyanobacterium synechocystis sp. PCC 6803

Naoya Shimada, Yukiko Okuda, Kaisei Maeda, Daisuke Umeno, Shinichi Takaichi, Masahiko Ikeuchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Heterologous production of a useful carotenoid astaxanthin was achieved in a cyanobacterium Synechocystis sp. PCC 6803 with the aid of marine bacterial genes. Astaxanthin and its intermediates emerged at high levels, whereas β-carotene and zeaxanthin disappeared in the strain. Total carote-noid accumulation was nearly two fold compared with wild type. The astaxanthin-producing strain was capable of only growing heterotrophically, which was likely due to the absence of β-carotene. Further enhanced accumulation was pursued by gene overexpression for possible rate-limiting steps in the biosynthesis pathway.

Original language	English
Pages (from-to)	116-120
Number of pages	5
Journal	Journal of General and Applied Microbiology
Volume	66
Issue number	2
DOIs	https://doi.org/10.2323/jgam.2020.01.003
Publication status	Published - 2020
Externally published	Yes

Keywords

Astaxanthin
Cyanobacteria
Metabolic engineering
Phototrophic grow

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.2323/jgam.2020.01.003

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title = "Astaxanthin production in a model cyanobacterium synechocystis sp. PCC 6803",

abstract = "Heterologous production of a useful carotenoid astaxanthin was achieved in a cyanobacterium Synechocystis sp. PCC 6803 with the aid of marine bacterial genes. Astaxanthin and its intermediates emerged at high levels, whereas β-carotene and zeaxanthin disappeared in the strain. Total carote-noid accumulation was nearly two fold compared with wild type. The astaxanthin-producing strain was capable of only growing heterotrophically, which was likely due to the absence of β-carotene. Further enhanced accumulation was pursued by gene overexpression for possible rate-limiting steps in the biosynthesis pathway.",

keywords = "Astaxanthin, Cyanobacteria, Metabolic engineering, Phototrophic grow",

author = "Naoya Shimada and Yukiko Okuda and Kaisei Maeda and Daisuke Umeno and Shinichi Takaichi and Masahiko Ikeuchi",

note = "Funding Information: This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science for Scientific Research (16H06558 to M.I.), and from JST for CREST program (JPMJCR1653 to M.I.). Publisher Copyright: {\textcopyright} 2020 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.",

year = "2020",

doi = "10.2323/jgam.2020.01.003",

language = "English",

volume = "66",

pages = "116--120",

journal = "Journal of General and Applied Microbiology",

issn = "0022-1260",

publisher = "Microbiology Research Foundation",

number = "2",

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TY - JOUR

T1 - Astaxanthin production in a model cyanobacterium synechocystis sp. PCC 6803

AU - Shimada, Naoya

AU - Okuda, Yukiko

AU - Maeda, Kaisei

AU - Umeno, Daisuke

AU - Takaichi, Shinichi

AU - Ikeuchi, Masahiko

N1 - Funding Information: This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science for Scientific Research (16H06558 to M.I.), and from JST for CREST program (JPMJCR1653 to M.I.). Publisher Copyright: © 2020 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.

PY - 2020

Y1 - 2020

N2 - Heterologous production of a useful carotenoid astaxanthin was achieved in a cyanobacterium Synechocystis sp. PCC 6803 with the aid of marine bacterial genes. Astaxanthin and its intermediates emerged at high levels, whereas β-carotene and zeaxanthin disappeared in the strain. Total carote-noid accumulation was nearly two fold compared with wild type. The astaxanthin-producing strain was capable of only growing heterotrophically, which was likely due to the absence of β-carotene. Further enhanced accumulation was pursued by gene overexpression for possible rate-limiting steps in the biosynthesis pathway.

AB - Heterologous production of a useful carotenoid astaxanthin was achieved in a cyanobacterium Synechocystis sp. PCC 6803 with the aid of marine bacterial genes. Astaxanthin and its intermediates emerged at high levels, whereas β-carotene and zeaxanthin disappeared in the strain. Total carote-noid accumulation was nearly two fold compared with wild type. The astaxanthin-producing strain was capable of only growing heterotrophically, which was likely due to the absence of β-carotene. Further enhanced accumulation was pursued by gene overexpression for possible rate-limiting steps in the biosynthesis pathway.

KW - Astaxanthin

KW - Cyanobacteria

KW - Metabolic engineering

KW - Phototrophic grow

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JF - Journal of General and Applied Microbiology

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ER -

Astaxanthin production in a model cyanobacterium synechocystis sp. PCC 6803

Abstract

Keywords

ASJC Scopus subject areas

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