ATP Photosynthetic vesicles for light-driven bioprocesses

Kiyotaka Y. Hara, Rie Suzuki, Toshiharu Suzuki, Masasuke Yoshida, Kuniki Kino*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TF0F1-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.

Original languageEnglish
Pages (from-to)1133-1138
Number of pages6
JournalBiotechnology Letters
Volume33
Issue number6
DOIs
Publication statusPublished - 2011 Jun

Keywords

  • ATP synthase
  • Membrane vesicle
  • Photosynthetic ATP synthesis
  • Rhodopsin
  • Vesicles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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