Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Hiroyuki Ota; Tae Kyu Lim; Tsuyoshi Tanaka; Tomoko Yoshino; Manabu Harada; Tadashi Matsunaga

doi:10.1016/j.jbiotec.2006.03.007

Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Hiroyuki Ota, Tae Kyu Lim, Tsuyoshi Tanaka, Tomoko Yoshino, Manabu Harada, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A₂₆₀-A₃₂₀):(A₂₈₀-A₃₂₀) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

Original language	English
Pages (from-to)	361-368
Number of pages	8
Journal	Journal of Biotechnology
Volume	125
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiotec.2006.03.007
Publication status	Published - 2006 Sept 18
Externally published	Yes

Keywords

Aminosilane-modified bacterial magnetic particles
DNA extraction
Genetically modified maize

ASJC Scopus subject areas

Biotechnology

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10.1016/j.jbiotec.2006.03.007

Cite this

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title = "Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles",

abstract = "A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.",

keywords = "Aminosilane-modified bacterial magnetic particles, DNA extraction, Genetically modified maize",

author = "Hiroyuki Ota and Lim, {Tae Kyu} and Tsuyoshi Tanaka and Tomoko Yoshino and Manabu Harada and Tadashi Matsunaga",

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doi = "10.1016/j.jbiotec.2006.03.007",

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T1 - Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

AU - Ota, Hiroyuki

AU - Lim, Tae Kyu

AU - Tanaka, Tsuyoshi

AU - Yoshino, Tomoko

AU - Harada, Manabu

AU - Matsunaga, Tadashi

PY - 2006/9/18

Y1 - 2006/9/18

N2 - A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

AB - A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

KW - Aminosilane-modified bacterial magnetic particles

KW - DNA extraction

KW - Genetically modified maize

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Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Abstract

Keywords

ASJC Scopus subject areas

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