Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Satoru Kurokawa; Jun Kabayama; Tsuguaki Fukuyasu; Seong Don Hwang; Chan Il Park; Seong Bin Park; Carmelo S. del Castillo; Jun ichi Hikima; Tae Sung Jung; Hidehiro Kondo; Ikuo Hirono; Haruko Takeyama; Takashi Aoki

doi:10.1007/s10126-012-9492-x

Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Satoru Kurokawa, Jun Kabayama, Tsuguaki Fukuyasu, Seong Don Hwang, Chan Il Park, Seong Bin Park, Carmelo S. del Castillo, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aoki^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

Original language	English
Pages (from-to)	340-348
Number of pages	9
Journal	Marine Biotechnology
Volume	15
Issue number	3
DOIs	https://doi.org/10.1007/s10126-012-9492-x
Publication status	Published - 2013 Jun

Keywords

MALDI Biotyper
Mycobacterium marinum
Mycobacterium sp.
Phylogenetic analysis

ASJC Scopus subject areas

Biotechnology
Aquatic Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10126-012-9492-x

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Kurokawa, S., Kabayama, J., Fukuyasu, T., Hwang, S. D., Park, C. I., Park, S. B., del Castillo, C. S., Hikima, J. I., Jung, T. S., Kondo, H., Hirono, I., Takeyama, H., & Aoki, T. (2013). Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System. Marine Biotechnology, 15(3), 340-348. https://doi.org/10.1007/s10126-012-9492-x

Kurokawa, S, Kabayama, J, Fukuyasu, T, Hwang, SD, Park, CI, Park, SB, del Castillo, CS, Hikima, JI, Jung, TS, Kondo, H, Hirono, I, Takeyama, H & Aoki, T 2013, 'Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System', Marine Biotechnology, vol. 15, no. 3, pp. 340-348. https://doi.org/10.1007/s10126-012-9492-x

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title = "Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System",

abstract = "Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.",

keywords = "MALDI Biotyper, Mycobacterium marinum, Mycobacterium sp., Phylogenetic analysis",

author = "Satoru Kurokawa and Jun Kabayama and Tsuguaki Fukuyasu and Hwang, {Seong Don} and Park, {Chan Il} and Park, {Seong Bin} and {del Castillo}, {Carmelo S.} and Hikima, {Jun ichi} and Jung, {Tae Sung} and Hidehiro Kondo and Ikuo Hirono and Haruko Takeyama and Takashi Aoki",

note = "Funding Information: This work was partially supported by a grant from the World Class University Program (R32-10253) funded by the Ministry of Education, Science and Technology, South Korea.",

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doi = "10.1007/s10126-012-9492-x",

language = "English",

volume = "15",

pages = "340--348",

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T1 - Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

AU - Kurokawa, Satoru

AU - Kabayama, Jun

AU - Fukuyasu, Tsuguaki

AU - Hwang, Seong Don

AU - Park, Chan Il

AU - Park, Seong Bin

AU - del Castillo, Carmelo S.

AU - Hikima, Jun ichi

AU - Jung, Tae Sung

AU - Kondo, Hidehiro

AU - Hirono, Ikuo

AU - Takeyama, Haruko

AU - Aoki, Takashi

N1 - Funding Information: This work was partially supported by a grant from the World Class University Program (R32-10253) funded by the Ministry of Education, Science and Technology, South Korea.

PY - 2013/6

Y1 - 2013/6

N2 - Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

AB - Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

KW - MALDI Biotyper

KW - Mycobacterium marinum

KW - Mycobacterium sp.

KW - Phylogenetic analysis

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Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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