Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei

Takayuki Kamei; Katsushi Suzuki; Kunio Shiota; Tomoya Ogawa; Michio Takahashi

doi:10.1262/jrd.39.25

Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei

Takayuki Kamei, Katsushi Suzuki, Kunio Shiota, Tomoya Ogawa, Michio Takahashi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-nuclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase I. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cel.

Original language	English
Pages (from-to)	25-31
Number of pages	7
Journal	Journal of Reproduction and Development
Volume	39
Issue number	1
DOIs	https://doi.org/10.1262/jrd.39.25
Publication status	Published - 1993
Externally published	Yes

Keywords

Basic fibroblast growth factor
DNA-binding
Nuclear localization
Testis

ASJC Scopus subject areas

Animal Science and Zoology

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10.1262/jrd.39.25

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title = "Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei",

abstract = "Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-nuclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase I. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cel.",

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author = "Takayuki Kamei and Katsushi Suzuki and Kunio Shiota and Tomoya Ogawa and Michio Takahashi",

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AU - Kamei, Takayuki

AU - Suzuki, Katsushi

AU - Shiota, Kunio

AU - Ogawa, Tomoya

AU - Takahashi, Michio

PY - 1993

Y1 - 1993

N2 - Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-nuclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase I. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cel.

AB - Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-nuclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase I. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cel.

KW - Basic fibroblast growth factor

KW - DNA-binding

KW - Nuclear localization

KW - Testis

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Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei

Abstract

Keywords

ASJC Scopus subject areas

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