Biocatalytic synthesis of dihydroxynaphthoic acids by cytochrome P450 CYP199A2

Toshiki Furuya, Kuniki Kino*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


CYP199A2, a bacterial P450 monooxygenase from Rhodopseudomonas palustris, was found to exhibit oxidation activity towards three hydroxynaphthoic acids. Whole cells of the recombinant Escherichia coli strain expressing CYP199A2 efficiently catalyzed the regioselective oxidation of 1-, 3-, and 6-hydroxy-2-naphthoic acids to produce 1,7-, 3,7-, and 6,7-dihydroxynaphthoic acid respectively. These results suggest that CYP199A2 might be a useful oxidation biocatalyst for the synthesis of dihydroxynaphthoic acids.

Original languageEnglish
Pages (from-to)2796-2799
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number12
Publication statusPublished - 2009


  • CYP199A2
  • Cytochrome p450
  • Hydroxylation
  • Naphthoic acid
  • Oxidation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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