Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay

Tomoko Yoshino*, Chihiro Kaji, Tadashi Matsunaga

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Magnetic particles are used for various biomedical applications because they are easy to both handle and separate from biological samples. Nano-sized bacterial magnetic particles (BacMPs) that display the human estrogen receptor ligand binding domain (ERLBD) on their surfaces were successfully produced by the magnetotactic bacterium, Magnetospirillum inagneticum AMB-1. A receptor assay for endocrine-disrupting chemicals using ERLBD- displaying BacMPs was developed. A BacMP membrane-specific protein, Mmsl6 or Mmsl3, was used as an anchor protein to localize the ERLBD on the surfaces of BacMPs. ERLBD- BacMP complexes were assayed for competitive binding of alkaline phosphatase- conjugated 17(3-estradiol (ALP-E2). Inhibition curve of ALP-E2 to the powerful antagonist, tamoxifen was generated by measuring decreases in luminescence intensity that resulted from the enzymatic reaction of alkaline phosphatase. The overall simplicity of this receptor-binding assay results in a method that can be easily adapted to a high-throughput format.

Original languageEnglish
Title of host publicationFrom Biological Materials to Biomimetic Material Synthesis
Number of pages6
Publication statusPublished - 2008
Externally publishedYes
Event2008 MRS Spring Meeting - San Francisco, CA
Duration: 2008 Mar 242008 Mar 28


Other2008 MRS Spring Meeting
CitySan Francisco, CA

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials


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