Abstract
Magnetic particles are used for various biomedical applications because they are easy to both handle and separate from biological samples. Nano-sized bacterial magnetic particles (BacMPs) that display the human estrogen receptor ligand binding domain (ERLBD) on their surfaces were successfully produced by the magnetotactic bacterium, Magnetospirillum inagneticum AMB-1. A receptor assay for endocrine-disrupting chemicals using ERLBD- displaying BacMPs was developed. A BacMP membrane-specific protein, Mmsl6 or Mmsl3, was used as an anchor protein to localize the ERLBD on the surfaces of BacMPs. ERLBD- BacMP complexes were assayed for competitive binding of alkaline phosphatase- conjugated 17(3-estradiol (ALP-E2). Inhibition curve of ALP-E2 to the powerful antagonist, tamoxifen was generated by measuring decreases in luminescence intensity that resulted from the enzymatic reaction of alkaline phosphatase. The overall simplicity of this receptor-binding assay results in a method that can be easily adapted to a high-throughput format.
| Original language | English |
|---|---|
| Title of host publication | From Biological Materials to Biomimetic Material Synthesis |
| Pages | 13-18 |
| Number of pages | 6 |
| Volume | 1094 |
| Publication status | Published - 2008 |
| Externally published | Yes |
| Event | 2008 MRS Spring Meeting - San Francisco, CA Duration: 2008 Mar 24 → 2008 Mar 28 |
Other
| Other | 2008 MRS Spring Meeting |
|---|---|
| City | San Francisco, CA |
| Period | 08/3/24 → 08/3/28 |
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering
- Mechanics of Materials