Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba; Yusuke Fujino; Tomoka Matsuda; Tetsuya Osaka; Ikuo Satoh

doi:10.1016/S0925-4005(98)00259-7

Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba, Yusuke Fujino, Tomoka Matsuda, Tetsuya Osaka^*, Ikuo Satoh

^*Corresponding author for this work

Research Organization for Nano & Life Innovation

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10^-7 to 1.0 × 10^-4 mol dm^-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10^-5 to 1.0 × 10^-3 mol dm^-3 with a sensitivity of 50 mV decade^-1.

Original language	English
Pages (from-to)	78-83
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	52
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4005(98)00259-7
Publication status	Published - 1998 Sept 15

Keywords

Ag(I) ion
Arginine
Biosensor
Polypyrrole
Urease

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/S0925-4005(98)00259-7

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title = "Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex",

abstract = "The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10-7 to 1.0 × 10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10-5 to 1.0 × 10-3 mol dm-3 with a sensitivity of 50 mV decade-1.",

keywords = "Ag(I) ion, Arginine, Biosensor, Polypyrrole, Urease",

author = "Shinichi Komaba and Yusuke Fujino and Tomoka Matsuda and Tetsuya Osaka and Ikuo Satoh",

note = "Funding Information: This work was financially supported by a grant-in-aid for Scientific Research (C), the Ministry of Education, Science, Sports and Culture of Japan. ",

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AU - Komaba, Shinichi

AU - Fujino, Yusuke

AU - Matsuda, Tomoka

AU - Osaka, Tetsuya

AU - Satoh, Ikuo

N1 - Funding Information: This work was financially supported by a grant-in-aid for Scientific Research (C), the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1998/9/15

Y1 - 1998/9/15

N2 - The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10-7 to 1.0 × 10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10-5 to 1.0 × 10-3 mol dm-3 with a sensitivity of 50 mV decade-1.

AB - The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10-7 to 1.0 × 10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10-5 to 1.0 × 10-3 mol dm-3 with a sensitivity of 50 mV decade-1.

KW - Ag(I) ion

KW - Arginine

KW - Biosensor

KW - Polypyrrole

KW - Urease

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Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Abstract

Keywords

ASJC Scopus subject areas

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