TY - JOUR
T1 - Biosensing by optical waveguide spectroscopy based on localized surface plasmon resonance of gold nanoparticles used as a probe or as a label
AU - Kajiura, Mayuki
AU - Nakanishi, Takuya
AU - Iida, Hironori
AU - Takada, Harumi
AU - Osaka, Tetsuya
N1 - Funding Information:
This work was carried out in the Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW) and supported in part by KAKENHI (20002006) of the Ministry of Education, Culture, Sports, Science and Technology, Japan. Assistance in manuscript preparation provided by Dr. Yutaka Okinaka is gratefully acknowledged.
PY - 2009/7/1
Y1 - 2009/7/1
N2 - The application of localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of biotin-streptavidin binding, as a typical biological reaction, was investigated by using optical waveguide spectroscopy, and two different modes for the use of gold nanoparticles, one as a probe and the other as a label were compared with each other. The combination with optical waveguide spectroscopy was found to bring about a high sensitivity for the biomolecular detection system using LSPR of gold nanoparticles in both modes. In particular, the mode using gold nanoparticles as a label was demonstrated to be of advantage to devising proper procedures for using nanoparticles and evaluating actual response relevant to the phenomenon concerned, and thus to sensitive detection.
AB - The application of localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of biotin-streptavidin binding, as a typical biological reaction, was investigated by using optical waveguide spectroscopy, and two different modes for the use of gold nanoparticles, one as a probe and the other as a label were compared with each other. The combination with optical waveguide spectroscopy was found to bring about a high sensitivity for the biomolecular detection system using LSPR of gold nanoparticles in both modes. In particular, the mode using gold nanoparticles as a label was demonstrated to be of advantage to devising proper procedures for using nanoparticles and evaluating actual response relevant to the phenomenon concerned, and thus to sensitive detection.
KW - Biotin-streptavidin reaction
KW - Gold nanoparticle
KW - Localized surface plasmon resonance
KW - Optical waveguide spectroscopy
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U2 - 10.1016/j.jcis.2009.03.016
DO - 10.1016/j.jcis.2009.03.016
M3 - Article
C2 - 19395015
AN - SCOPUS:67349236776
SN - 0021-9797
VL - 335
SP - 140
EP - 145
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
IS - 1
ER -