TY - JOUR
T1 - BSA and lysozyme adsorption on homoionic montmorillonite
T2 - Influence of the interlayer cation
AU - Lepoitevin, Mathilde
AU - Jaber, Maguy
AU - Guégan, Régis
AU - Janot, Jean Marc
AU - Dejardin, Philippe
AU - Henn, François
AU - Balme, Sébastien
PY - 2014/6
Y1 - 2014/6
N2 - This study aims to investigate the influence of interlayer cation alkali metal series (Li+, Na+, K+, Rb+, Cs+) and alkali earth metal (Mg2+, Ca2+, Ba2+) on adsorption of model proteins (lysozyme and bovine serum albumin). The localizations of both proteins and their conformational modifications in the interlayer space of the natural clay mineral were studied by X-ray diffraction, transmission electronic microscopy and fluorescence experiments. Based on Langmuir model isotherms, a strong influence of interlayer cation on maximum adsorbed amount and adsorption equilibrium constant has been observed. However the usual kosmotrope/chaotrope classification cannot be used to describe protein adsorption.
AB - This study aims to investigate the influence of interlayer cation alkali metal series (Li+, Na+, K+, Rb+, Cs+) and alkali earth metal (Mg2+, Ca2+, Ba2+) on adsorption of model proteins (lysozyme and bovine serum albumin). The localizations of both proteins and their conformational modifications in the interlayer space of the natural clay mineral were studied by X-ray diffraction, transmission electronic microscopy and fluorescence experiments. Based on Langmuir model isotherms, a strong influence of interlayer cation on maximum adsorbed amount and adsorption equilibrium constant has been observed. However the usual kosmotrope/chaotrope classification cannot be used to describe protein adsorption.
KW - Homoionic-clay
KW - Langmuir isotherm
KW - Montmorillonite
KW - Protein adsorption
UR - http://www.scopus.com/inward/record.url?scp=84902112996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902112996&partnerID=8YFLogxK
U2 - 10.1016/j.clay.2014.05.003
DO - 10.1016/j.clay.2014.05.003
M3 - Article
AN - SCOPUS:84902112996
SN - 0169-1317
VL - 95
SP - 396
EP - 402
JO - Applied Clay Science
JF - Applied Clay Science
ER -