Cell cycle and size sorting of mammalian cells using a microfluidic device

Satoshi Migita; Kei Funakoshi; Daiju Tsuya; Tomohiko Yamazaki; Akiyoshi Taniguchi; Yoshimasa Sugimoto; Nobutaka Hanagata; Toshiyuki Ikoma

doi:10.1039/c0ay00039f

Cell cycle and size sorting of mammalian cells using a microfluidic device

Satoshi Migita, Kei Funakoshi, Daiju Tsuya, Tomohiko Yamazaki, Akiyoshi Taniguchi, Yoshimasa Sugimoto, Nobutaka Hanagata, Toshiyuki Ikoma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Microfluidic devices can sort viable mammalian cells by size. In this study, we investigated size-based sorting of cells using flow splitting microfluidic devices based on hydrodynamic filtration for noninvasive cell cycle synchronization. Two different types of mammalian cell lines, HepG2 (human hepatocellular liver carcinoma cell line) and NIH/3T3 (mouse embryonic fibroblast cell line) were sorted by microfluidic device and its DNA contents were analyzed. Our results showed that a microfluidic device can synchronize the cell cycle after size separation. The damage-free separation of living cells in different phases of the cell cycle represents a potentially promising technology for the investigation of gene transfection and gene expression.

Original language	English
Pages (from-to)	657-660
Number of pages	4
Journal	Analytical Methods
Volume	2
Issue number	6
DOIs	https://doi.org/10.1039/c0ay00039f
Publication status	Published - 2010 Jun
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Chemical Engineering(all)
Engineering(all)

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abstract = "Microfluidic devices can sort viable mammalian cells by size. In this study, we investigated size-based sorting of cells using flow splitting microfluidic devices based on hydrodynamic filtration for noninvasive cell cycle synchronization. Two different types of mammalian cell lines, HepG2 (human hepatocellular liver carcinoma cell line) and NIH/3T3 (mouse embryonic fibroblast cell line) were sorted by microfluidic device and its DNA contents were analyzed. Our results showed that a microfluidic device can synchronize the cell cycle after size separation. The damage-free separation of living cells in different phases of the cell cycle represents a potentially promising technology for the investigation of gene transfection and gene expression.",

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AU - Migita, Satoshi

AU - Funakoshi, Kei

AU - Tsuya, Daiju

AU - Yamazaki, Tomohiko

AU - Taniguchi, Akiyoshi

AU - Sugimoto, Yoshimasa

AU - Hanagata, Nobutaka

AU - Ikoma, Toshiyuki

PY - 2010/6

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AB - Microfluidic devices can sort viable mammalian cells by size. In this study, we investigated size-based sorting of cells using flow splitting microfluidic devices based on hydrodynamic filtration for noninvasive cell cycle synchronization. Two different types of mammalian cell lines, HepG2 (human hepatocellular liver carcinoma cell line) and NIH/3T3 (mouse embryonic fibroblast cell line) were sorted by microfluidic device and its DNA contents were analyzed. Our results showed that a microfluidic device can synchronize the cell cycle after size separation. The damage-free separation of living cells in different phases of the cell cycle represents a potentially promising technology for the investigation of gene transfection and gene expression.

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Cell cycle and size sorting of mammalian cells using a microfluidic device

Abstract

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