Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery

Hideaki Matsubayashi; Yutetsu Kuruma; Takuya Ueda

doi:10.1007/s11084-014-9389-y

Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery

Hideaki Matsubayashi, Yutetsu Kuruma, Takuya Ueda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.

Original language	English
Pages (from-to)	331-334
Number of pages	4
Journal	Origins of Life and Evolution of Biospheres
Volume	44
Issue number	4
DOIs	https://doi.org/10.1007/s11084-014-9389-y
Publication status	Published - 2014 Dec 1
Externally published	Yes

Keywords

Cell-free protein synthesis system
Liposome
Membrane protein
SecYEG translocon

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Space and Planetary Science

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10.1007/s11084-014-9389-y

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abstract = "The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.",

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AU - Matsubayashi, Hideaki

AU - Kuruma, Yutetsu

AU - Ueda, Takuya

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AB - The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.

KW - Cell-free protein synthesis system

KW - Liposome

KW - Membrane protein

KW - SecYEG translocon

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Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery

Abstract

Keywords

ASJC Scopus subject areas

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