Abstract
In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.
| Original language | English |
|---|---|
| Pages | 215-217 |
| Number of pages | 3 |
| Publication status | Published - 2008 |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16 |
Conference
| Conference | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country/Territory | United States |
| City | San Diego, CA |
| Period | 08/10/12 → 08/10/16 |
Keywords
- Cell rupture
- Lysosome
- Microvalve
- Mitochondria
- Nano needle array
- Organelle
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering