Cellular responses to electrochemical killing process by applying a constant potential in synchronously cultured Saccharomyces Cerevisiae

Haruko Takeyama; Takeyuki Suzuki; Yoshiko Okamura; Tetsushi Mori; Tadashi Matsunaga

doi:10.5796/electrochemistry.76.603

Cellular responses to electrochemical killing process by applying a constant potential in synchronously cultured Saccharomyces Cerevisiae

Haruko Takeyama^*, Takeyuki Suzuki, Yoshiko Okamura, Tetsushi Mori, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

To elucidate the mechanism of the electrochemical killing, the global gene expression of synchronously cultured Saccharomyces cerevisiae exposed to electrochemical stimulus was analyzed using DNA microarray. By applying a potential of 1.0 V vs. saturated calomel electrode (SCE), oxidative stress related genes were expressed after only 5 minutes. The gene for cta1 exhibited the highest expression level, while ROS gradually accumulated inside the cells. Genes for signal transduction which may be related to cellular apoptosis were also seen expressed.

Original language	English
Pages (from-to)	603-605
Number of pages	3
Journal	Electrochemistry
Volume	76
Issue number	8
DOIs	https://doi.org/10.5796/electrochemistry.76.603
Publication status	Published - 2008 Aug
Externally published	Yes

Keywords

Electrochemical killing process
Synchronous culture
Transcriptome

ASJC Scopus subject areas

Electrochemistry

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10.5796/electrochemistry.76.603

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abstract = "To elucidate the mechanism of the electrochemical killing, the global gene expression of synchronously cultured Saccharomyces cerevisiae exposed to electrochemical stimulus was analyzed using DNA microarray. By applying a potential of 1.0 V vs. saturated calomel electrode (SCE), oxidative stress related genes were expressed after only 5 minutes. The gene for cta1 exhibited the highest expression level, while ROS gradually accumulated inside the cells. Genes for signal transduction which may be related to cellular apoptosis were also seen expressed.",

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author = "Haruko Takeyama and Takeyuki Suzuki and Yoshiko Okamura and Tetsushi Mori and Tadashi Matsunaga",

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AU - Takeyama, Haruko

AU - Suzuki, Takeyuki

AU - Okamura, Yoshiko

AU - Mori, Tetsushi

AU - Matsunaga, Tadashi

PY - 2008/8

Y1 - 2008/8

N2 - To elucidate the mechanism of the electrochemical killing, the global gene expression of synchronously cultured Saccharomyces cerevisiae exposed to electrochemical stimulus was analyzed using DNA microarray. By applying a potential of 1.0 V vs. saturated calomel electrode (SCE), oxidative stress related genes were expressed after only 5 minutes. The gene for cta1 exhibited the highest expression level, while ROS gradually accumulated inside the cells. Genes for signal transduction which may be related to cellular apoptosis were also seen expressed.

AB - To elucidate the mechanism of the electrochemical killing, the global gene expression of synchronously cultured Saccharomyces cerevisiae exposed to electrochemical stimulus was analyzed using DNA microarray. By applying a potential of 1.0 V vs. saturated calomel electrode (SCE), oxidative stress related genes were expressed after only 5 minutes. The gene for cta1 exhibited the highest expression level, while ROS gradually accumulated inside the cells. Genes for signal transduction which may be related to cellular apoptosis were also seen expressed.

KW - Electrochemical killing process

KW - Synchronous culture

KW - Transcriptome

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Cellular responses to electrochemical killing process by applying a constant potential in synchronously cultured Saccharomyces Cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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