TY - JOUR
T1 - Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease
AU - Komiyama, Makoto
AU - Takahashi, Yota
AU - Takeda, Naoya
AU - Matsumura, Kazunari
AU - Kodama, Teruyuki
N1 - Funding Information:
The authors should like to thank Profs. Peter B. Dervan and Anthony J. Kirby for their encouraging discussion. This work was partially supported by a Grant-in-Aid for Scientific Research in Priority Area from the Ministry of Education, Science, and Culture and by Nissan Science Foundation,
PY - 1994/1
Y1 - 1994/1
N2 - Cerium(IV) ion is attached to the 5′ of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidyly1(3′-5)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.
AB - Cerium(IV) ion is attached to the 5′ of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidyly1(3′-5)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.
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U2 - 10.1080/10610279408029859
DO - 10.1080/10610279408029859
M3 - Article
AN - SCOPUS:0002421061
SN - 1061-0278
VL - 4
SP - 31
EP - 34
JO - Supramolecular Chemistry
JF - Supramolecular Chemistry
IS - 1
ER -