Changes in expression of NMDA receptor subunit mRNA by perinatal exposure to dioxin

Masaki Kakeyama; Hideko Sone; Chiharu Tohyama

doi:10.1097/00001756-200112210-00031

Changes in expression of NMDA receptor subunit mRNA by perinatal exposure to dioxin

Masaki Kakeyama^*, Hideko Sone, Chiharu Tohyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Since dioxin and related compounds are suspected of affecting permanently the brain function of offspring of human and experimental animals, effects of perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of rat NMDA receptor NR2A and NR2B subunit mRNA were examined. The mRNA quantification by competitive RT-PCR clearly revealed that TCDD inhibited NR2B mRNA expression and enhanced NR2A mRNA expression in the neocortex and hippocampus on postnatal day (PND) 49, whereas these changes in mRNA expression were not found on PND 5. The results demonstrate for the first time that the perinatal exposure to TCDD can alter the molecular basis of brain of offspring in adulthood.

Original language	English
Pages (from-to)	4009-4012
Number of pages	4
Journal	NeuroReport
Volume	12
Issue number	18
DOIs	https://doi.org/10.1097/00001756-200112210-00031
Publication status	Published - 2001 Dec 21
Externally published	Yes

Keywords

Competitive RT-PCR
Dioxin
NMDA receptor

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200112210-00031

Cite this

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abstract = "Since dioxin and related compounds are suspected of affecting permanently the brain function of offspring of human and experimental animals, effects of perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of rat NMDA receptor NR2A and NR2B subunit mRNA were examined. The mRNA quantification by competitive RT-PCR clearly revealed that TCDD inhibited NR2B mRNA expression and enhanced NR2A mRNA expression in the neocortex and hippocampus on postnatal day (PND) 49, whereas these changes in mRNA expression were not found on PND 5. The results demonstrate for the first time that the perinatal exposure to TCDD can alter the molecular basis of brain of offspring in adulthood.",

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T1 - Changes in expression of NMDA receptor subunit mRNA by perinatal exposure to dioxin

AU - Kakeyama, Masaki

AU - Sone, Hideko

AU - Tohyama, Chiharu

PY - 2001/12/21

Y1 - 2001/12/21

N2 - Since dioxin and related compounds are suspected of affecting permanently the brain function of offspring of human and experimental animals, effects of perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of rat NMDA receptor NR2A and NR2B subunit mRNA were examined. The mRNA quantification by competitive RT-PCR clearly revealed that TCDD inhibited NR2B mRNA expression and enhanced NR2A mRNA expression in the neocortex and hippocampus on postnatal day (PND) 49, whereas these changes in mRNA expression were not found on PND 5. The results demonstrate for the first time that the perinatal exposure to TCDD can alter the molecular basis of brain of offspring in adulthood.

AB - Since dioxin and related compounds are suspected of affecting permanently the brain function of offspring of human and experimental animals, effects of perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of rat NMDA receptor NR2A and NR2B subunit mRNA were examined. The mRNA quantification by competitive RT-PCR clearly revealed that TCDD inhibited NR2B mRNA expression and enhanced NR2A mRNA expression in the neocortex and hippocampus on postnatal day (PND) 49, whereas these changes in mRNA expression were not found on PND 5. The results demonstrate for the first time that the perinatal exposure to TCDD can alter the molecular basis of brain of offspring in adulthood.

KW - Competitive RT-PCR

KW - Dioxin

KW - NMDA receptor

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Changes in expression of NMDA receptor subunit mRNA by perinatal exposure to dioxin

Abstract

Keywords

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