Characteristics of bacteria showing high denitrification activity in saline wastewater

S. Yoshie, T. Ogawa, H. Makino, H. Hirosawa, S. Tsuneda*, A. Hirata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Aims: Denitrification efficiency at 10% salinity was compared with that at 2% salinity. The characteristics of bacterial strains isolated from the denitrification system, where an improvement of denitrification efficiency was observed at a high salinity were investigated. Methods and Results: Two continuous feeding denitrification systems for saline solutions of 2% and 10% salinity, were operated. Denitrification efficiency at 10% salinity was higher than that at 2% salinity. The bacterial strains were isolated using the trypticase soy agar (TSA) medium at 30°C. The phylogenetic analysis of 16S rRNA gene sequences of isolates indicated that halophilic species were predominant at 10% salinity. Conclusions: The improvement of denitrification efficiency at a high salinity was demonstrated. The strains isolated from the denitrifying system with 10% salinity were halophilic bacteria, Halomonas sp. and Marinobacter sp., suggesting that these bacteria show a high denitrifying activity at 10% salinity. Significance and Impact of the Study: The long-term acclimated sludge used in this study resulted in high denitrification performance at a high salinity, indicating that the design of a high-performance denitrification system for saline wastewater will be possible.

Original languageEnglish
Pages (from-to)277-283
Number of pages7
JournalLetters in Applied Microbiology
Issue number3
Publication statusPublished - 2006 Mar


  • Halomonas
  • Halophilic denitrifying bacteria
  • Improvement of denitrification efficiency
  • Isolation
  • Marinobacter
  • Wastewater treatment system

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology


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