TY - JOUR
T1 - Characteristics of hb-vesicles and encapsulation procedure
AU - Takeoka, S.
AU - Sakai, H.
AU - Terase, K.
AU - Nishide, H.
AU - Tsuchida, E.
PY - 1994/1
Y1 - 1994/1
N2 - The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca. pH 7, that would relate to the isoelectric point (pl) of Hb at 25 C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicles encapsulating 40 g/dl Hb with only one bilayer membrane were isolated by using the difference in the density of the vesicles.
AB - The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca. pH 7, that would relate to the isoelectric point (pl) of Hb at 25 C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicles encapsulating 40 g/dl Hb with only one bilayer membrane were isolated by using the difference in the density of the vesicles.
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U2 - 10.3109/10731199409117922
DO - 10.3109/10731199409117922
M3 - Article
C2 - 7994410
AN - SCOPUS:0028141652
SN - 1073-1199
VL - 22
SP - 861
EP - 866
JO - Artificial Cells, Blood Substitutes, and Biotechnology
JF - Artificial Cells, Blood Substitutes, and Biotechnology
IS - 3
ER -