Characteristics of hb-vesicles and encapsulation procedure

S. Takeoka, H. Sakai, K. Terase, H. Nishide, E. Tsuchida

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The performance of Hb-vesicles depends on the weight ratio of Hb to lipid ([Hb]/[Lipid]). This value is improved by lowering the number of bilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained at ca. pH 7, that would relate to the isoelectric point (pl) of Hb at 25 C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicles encapsulating 40 g/dl Hb with only one bilayer membrane were isolated by using the difference in the density of the vesicles.

Original languageEnglish
Pages (from-to)861-866
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Issue number3
Publication statusPublished - 1994 Jan

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering


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